study of paraoxonase -1 gene polymorphism in a healthy population of

human serum paraoxonase (hupon1:   ec 3.1.8.1), a calcium-dependent esterase, is synthesized in the liver and   widely distributed in tissues including liver, kidney, intestine, and serum,   where it is associated exclusively with high-density lipoprotein.  human paraoxonase-1 plays an important role   in prevention of atherosclerosis and also protection against organophosphate-induced   neurotoxicity. paraoxonase-1 shows 2 common polymorphisms: q/r at position   192 and m/l at position 55. in this study, paraoxonase-1 192 and 55   polymorphisms were investigated in 64 healthy iranian individuals. genomic   dna was isolated from whole blood by the bartlett method, and paraoxonase-1 genotypes   were determined by polymerase chain   reaction amplification followed by restriction isotyping and gel   electrophoresis. the chi-square test was used to evaluate the hardy-weinberg   equilibrium. the genotype frequencies for paraoxonase 1-q192r­ were   approximately 47% (qq), 41% (qr) and 12% (rr) and for paraoxonase-1 m55l, 44%   (ll), 44% (ml) and 12% (mm). thus, the frequency of alleles r, l, q, and m   were 0.33, 0.66, 0.67, and 0.34   respectively. in conclusion, the frequencies of paraoxonase-1 192 and 55   polymorphisms in this group of iranian population were different from those   seen in other asian populations from japan and china but similar to european   (caucasians).